Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
2 ARTICLES PUBLISHED IN JoVE
Biology
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments
Matteus Krappitz 1, Christoph Korbmacher 1, Silke Haerteis 1
1Institut für Zelluläre und Molekulare Physiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Proteolytic activation of the epithelial sodium channel (ENaC) heterologously expressed in Xenopus laevis oocytes can be demonstrated by combining current measurements with a biotinylation approach to investigate the appearance of ion channel cleavage products at the cell surface. Functionally important cleavage sites can be identified by using site-directed mutagenesis.
Education
Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions
José R. Agudo 1, Jiwon Han 1, Jinyoung Park 1, Sinyoung Kwon 1, Soebiakto Loekman 2, Giovanni Luzi 1, Christoph Linderberger 3, Antonio Delgado 1,4, Andreas Wierschem 4
1Institute of Fluid Mechanics, FAU Busan Campus, University of Erlangen-Nuremberg, 2Institute of Chemical Reaction Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 3Institute of Bioprocess Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 4Institute of Fluid Mechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Two different methods for characterizing the incipient particle motion of a single bead as a function of the sediment bed geometry from laminar to turbulent flow are presented.
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados