U661, Montpellier
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Primary Culture of Mouse Dopaminergic Neurons
Florence Gaven 1,2,3, Philippe Marin 1,2,3, Sylvie Claeysen 1,2,3
1CNRS, UMR-5203, Institut de Génomique Fonctionnelle, Montpellier, 2Inserm, U661, Montpellier, 3Universités de Montpellier
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.
Medicine
Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases
Margarita Arango-Lievano 1,2,3, Patrizia Giannoni 1,2,3, Sylvie Claeysen 1,2,3, Nicola Marchi 1,2,3, Freddy Jeanneteau 1,2,3
1Inserm, U1191, Institute of Functional Genomics, 2CNRS, UMR-5203, 3Université de Montpellier
This manuscript describes a procedure to track the remodeling of the cerebrovasculature during amyloid plaque accumulation in vivo using longitudinal two-photon microscopy. A thinned-skull preparation enables the visualization of fluorescent dyes to assess the progression of cerebrovascular damage in a mouse model of Alzheimer's disease.
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