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Primary Culture of Mouse Dopaminergic Neurons

DOI :

10.3791/51751-v

September 8th, 2014

September 8th, 2014

36,405 Views

1CNRS, UMR-5203, Institut de Génomique Fonctionnelle, Montpellier, 2Inserm, U661, Montpellier, 3Universités de Montpellier

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

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Mouse Dopaminergic Neurons

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