IFOM, FIRC Institute of Molecular Oncology

1 ARTICLES PUBLISHED IN JoVE

Medicine

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Benedetta Apollonio^1,2, Maria Teresa Sabrina Bertilaccio¹, Umberto Restuccia³, Pamela Ranghetti¹, Federica Barbaglio¹, Paolo Ghia^1,4, Federico Caligaris-Cappio^1,4, Cristina Scielzo¹

¹Division of Molecular Oncology, IRCCS, San Raffaele Scientific Institute, ²Department of Haemato-Oncology, King's College London, ³IFOM, FIRC Institute of Molecular Oncology, ⁴Università Vita-Salute San Raffaele

Here we describe a protocol that couples two proteomic techniques, namely 2-dimensional Electrophoresis (2DE) and Mass Spectrometry (MS), to identify differentially expressed/post-translational modified proteins among two or more groups of primary samples. This approach, together with functional experiments, allows the identification and characterization of prognostic markers/therapeutic targets.