From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic LeukemiaBenedetta Apollonio 1,2, Maria Teresa Sabrina Bertilaccio 1, Umberto Restuccia 3, Pamela Ranghetti 1, Federica Barbaglio 1, Paolo Ghia 1,4, Federico Caligaris-Cappio 1,4, Cristina Scielzo 1
1Division of Molecular Oncology, IRCCS, San Raffaele Scientific Institute, 2Department of Haemato-Oncology, King's College London, 3IFOM, FIRC Institute of Molecular Oncology, 4Università Vita-Salute San Raffaele
Here we describe a protocol that couples two proteomic techniques, namely 2-dimensional Electrophoresis (2DE) and Mass Spectrometry (MS), to identify differentially expressed/post-translational modified proteins among two or more groups of primary samples. This approach, together with functional experiments, allows the identification and characterization of prognostic markers/therapeutic targets.
