Sorbonne Paris Cité

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Laura Megrelis^1,2,3, Jérôme Delon^1,2,3

¹Inserm U1016, Institut Cochin, ²Cnrs, UMR8104, ³Université Paris Descartes, Sorbonne Paris Cité

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

JoVE Journal

Increasing Pulmonary Artery Pulsatile Flow Improves Hypoxic Pulmonary Hypertension in Piglets

Audrey Courboulin¹, Chantal Kang², Olivier Baillard^3,4,5, Sebastien Bonnet¹, Pierre Bonnet⁶

¹Department of Medicine, Pulmonary Hypertension Research Group (CRIUCPQ), Laval University, ²Institut National de la Recherche Agronomique, ³Université Diderot Paris, Sorbonne Paris Cité, ⁴Hôpital Lariboisière, Physiologie clinique Explorations Fonctionnelles, ⁵INSERM U 965, ⁶Service de Cardiologie, Centre Hospitalier Universitaire Tours

Pulmonary hypertension is associated with a significant reduction in pulmonary artery pulsatility, contractility and elasticity, contributing to an increase in pulmonary artery pressure and pulmonary resistance. Using a hypoxic piglet model, this study demonstrated that improving pulmonary artery plasticity using a newly developed pulsatile catheter improves hypoxic pulmonary hypertension.