University of Colorado, Anschutz Medical Campus
2 ARTICLES PUBLISHED IN JoVE
Medicine
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Touraj Shokati 1, Nicholas Bodenberger 1, Holly Gadpaille 1, Björn Schniedewind 1, Alexander A. Vinks 2, Wenlei Jiang 3, Rita R. Alloway 4, Uwe Christians 1
1iC42 Clinical Research and Development, University of Colorado, Anschutz Medical Campus, 2Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, 3Food and Drug Administration (FDA), Center of Drug Evaluation Research - Office of Generic Drugs, 4Transplant Clinical Research, University of Cincinnati
Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.
Biochemistry
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
Nicholas Bodenberger 1, Dennis Kubiczek 1, Frank Rosenau 1
1Center for Peptide Pharmaceuticals, Faculty of Natural Science, Ulm University
Different methods to manipulate three-dimensional architecture in protein-based hydrogels are evaluated here with respect to material properties. The macroporous networks are functionalized with a cell-adhesive peptide, and their feasibility in cell culture is evaluated using two different model cell lines.
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