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University of Colorado, Anschutz Medical Campus

2 ARTICLES PUBLISHED IN JoVE

Medicine

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Touraj Shokati¹, Nicholas Bodenberger¹, Holly Gadpaille¹, Björn Schniedewind¹, Alexander A. Vinks², Wenlei Jiang³, Rita R. Alloway⁴, Uwe Christians¹

¹iC42 Clinical Research and Development, University of Colorado, Anschutz Medical Campus, ²Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, ³Food and Drug Administration (FDA), Center of Drug Evaluation Research - Office of Generic Drugs, ⁴Transplant Clinical Research, University of Cincinnati

Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.

Biochemistry

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Nicholas Bodenberger¹, Dennis Kubiczek¹, Frank Rosenau¹

¹Center for Peptide Pharmaceuticals, Faculty of Natural Science, Ulm University

Different methods to manipulate three-dimensional architecture in protein-based hydrogels are evaluated here with respect to material properties. The macroporous networks are functionalized with a cell-adhesive peptide, and their feasibility in cell culture is evaluated using two different model cell lines.