University of Michigan Health System
3 ARTICLES PUBLISHED IN JoVE
Medicine
Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Sujay S. Galen 1, Darren R. Guffey 2, Jared W. Coburn 3, Moh H. Malek 1
1Physical Therapy Program and Integrative Physiology of Exercise Laboratory, Department of Health Care Sciences, Wayne State University, 2Sports Medicine and Physical Therapy, MEDSPORT, University of Michigan Health System, 3Department of Kinesiology, California State University, Fullerton
This protocol describes the electromyographic fatigue threshold which demarcates between nonfatiguing and fatiguing exercise workloads. This information could be used to develop a more individualized training program.
Medicine
Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System
Anthony N. Hage 1, Will Krause 1, Angela Mathues 1, Luke Krasner 2, Seth Kasten 1, Jonathan L. Eliason 1,3, Abhijit Ghosh 1
1Jobst Vascular Research Laboratory, University of Michigan Medical School, 2Department of Engineering, Purdue University, 3Department of Surgery, Section of Vascular Surgery, University of Michigan Health System
This protocol describes a method for exposing rodents to electronic cigarette vapor (E-vapor) and cigarette smoke. Exposure chambers are constructed by modifying anesthesia chambers with an automated pumping system that delivers E-vapor or cigarette smoke to rodents. This system can easily be modified to accommodate many experimental endpoints.
JoVE Core
Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands
Isabella Finco 1, Gary D. Hammer 1,2,3,4,5
1Department of Internal Medicine (Division of Metabolism, Endocrinology, and Diabetes), University of Michigan Health System, 2Department of Cell and Developmental Biology, University of Michigan Health System, 3Department of Molecular and Integrative Physiology, University of Michigan Health System, 4Endocrine Oncology Program, University of Michigan Health System, 5Comprehensive Cancer Center, University of Michigan Health System
Here we present a method to isolate adrenal glands from mice, fix the tissues, section them, and perform immunofluorescence staining.
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