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University of North Carolina - Chapel Hill

2 ARTICLES PUBLISHED IN JoVE

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Biology

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
Diego Bohórquez 1, Fariha Haque 2, Satish Medicetty 3, Rodger A. Liddle 1
1Department of Medicine, Duke University Medical Center, 2Department of Chemistry, University of North Carolina - Chapel Hill, 3Renovo Neural Incorporated

Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.

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Neuroscience

Assessment of Sensory Thresholds in Dogs Using Mechanical and Hot Thermal Quantitative Sensory Testing
Rachael M. Cunningham 1,2, Rachel M. Park 1,2, David Knazovicky 3, B. Duncan X. Lascelles 2,4,5,6, Margaret E. Gruen 1,2,4
1Comparative Behavioral Research, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 2Translational Research in Pain (TRiP) Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 3Small Animal Orthopedic Surgery, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4Comparative Pain Research and Education Center, College of Veterinary Medicine, North Carolina State University, 5Thurston Arthritis Center, UNC School of Medicine, University of North Carolina - Chapel Hill, 6Center for Translational Pain Research, Department of Anesthesiology, Duke University

This work describes a standard protocol for mechanical and hot thermal quantitative sensory testing to evaluate the somatosensory system in dogs. Sensory thresholds are measured using an electronic von Frey anesthesiometer, pressure algometer, and hot contact thermode.

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