University of North Carolina - Chapel Hill

2 ARTICLES PUBLISHED IN JoVE

Biology

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Diego Bohórquez¹, Fariha Haque², Satish Medicetty³, Rodger A. Liddle¹

¹Department of Medicine, Duke University Medical Center, ²Department of Chemistry, University of North Carolina - Chapel Hill, ³Renovo Neural Incorporated

Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.

Neuroscience

Assessment of Sensory Thresholds in Dogs Using Mechanical and Hot Thermal Quantitative Sensory Testing

Rachael M. Cunningham^1,2, Rachel M. Park^1,2, David Knazovicky³, B. Duncan X. Lascelles^2,4,5,6, Margaret E. Gruen^1,2,4

¹Comparative Behavioral Research, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, ²Translational Research in Pain (TRiP) Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, ³Small Animal Orthopedic Surgery, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, ⁴Comparative Pain Research and Education Center, College of Veterinary Medicine, North Carolina State University, ⁵Thurston Arthritis Center, UNC School of Medicine, University of North Carolina - Chapel Hill, ⁶Center for Translational Pain Research, Department of Anesthesiology, Duke University

This work describes a standard protocol for mechanical and hot thermal quantitative sensory testing to evaluate the somatosensory system in dogs. Sensory thresholds are measured using an electronic von Frey anesthesiometer, pressure algometer, and hot contact thermode.