DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance
2 ARTICLES PUBLISHED IN JoVE
Medicine
Heterotopic Cervical Heart Transplantation in Mice
Thomas Ratschiller 1,4, Marcus-André Deutsch 1, Julia Calzada-Wack 2, Frauke Neff 2, Christiane Roesch 3, Ralf Guenzinger 3, Ruediger Lange 3, Markus Krane 3
1Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, 2Institute of Pathology, Helmholtz Zentrum Munich, 3Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, 4Department of Cardiovascular and Thoracic Surgery, General Hospital Linz
The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.
Biology
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
Harald Lahm *1, Stefanie A. Doppler *1, Martina Dreßen 1, Klaudia Adamczyk 1, Marcus-André Deutsch 1, Hanna Ulrich 2, Matthias Schiemann 2,3, Rüdiger Lange 1,4, Markus Krane 1,4
1Department of Cardiovascular Surgery, German Heart Center Munich, Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, 3Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg), Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance
This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.
