Name: heterotopic cervical heart transplantation in mice
Uploaded: 2015-08-25T14:00:00.000+00:00
Duration: 9 min 48 s
Description: Watch this Scientific Journal Video about Heterotopic Cervical Heart Transplantation in Mice at JoVE.com

M.-A. Deutsch is supported by Dr. Rusche Forschungsprojekt (2014) Deutsche Stiftung f&#252;r Herzforschung and Deutsche Gesellschaft f&#252;r Thorax- Herz- und Gef&#228;&#223;chirurgie. M. Krane is supported by Deutsche Stiftung f&#252;r Herzforschung (F/37/11), Deutsches Zentrum f&#252;r Herz Kreislauf Forschung (DZHK B 13-050A; DZHK B 14-013SE) and Deutsche Forschungsgemeinschaft (KR3770/7-1; KR3770/9-1)

所有实验均批准了德国心脏中心慕尼黑和德国巴伐利亚州（GZ。55.2-1-54-2532.3-17-13）的政府。动物饲养在无特定病原体设施，并与"实验动物保健的原则"，由国家红会的医学研究和"指南实验动物的护理和使用"由美国国家科学院提供的配方获得人文关怀符合科学和美国国立卫生研究院（NIH公开号86-23，1985年修订）出版。 注:一套完整的无菌显微仪器，其中包括两个弯钳和血管扩张器，是必要的过程（ 图2a）。围手术期抗生素预防是不是经常必要的，但可能在免疫功能低下的动物来表示。使用终端异氟烷吸入处死动物。 供体的制备与移植收获 &lt;OL&gt; <li>麻醉通过腹膜内（IP）的动物注射咪达唑仑（5.0毫克/千克），美托咪定（0.5毫克/千克）和芬太尼（0.05毫克/千克体重）。鼠标放置在上术野仰卧位置和确认手术耐受由踏板撤回反射。 </li><li>皮肤消毒三次氯己定和执行中线腹部切口。缩回用湿纱布肠内向左以暴露下腔静脉。 </li><li>注射50 IU肝素通过下腔静脉抗凝稀释0.4ml的生理盐水。切开肝素化后的容器1分钟。 </li><li>打开隔膜和执行双边开胸，露出心脏。前胸壁被优反射并在适当位置的夹紧保持。 </li><li>取出胸腺和直言解剖主动脉和肺动脉主干之间的结缔组织。切开右上腔静脉。 </li><li>穿刺动员升主动脉处的水平装在一个5毫升注射器头臂干与地下30针并缓慢灌注心脏retrogradually而没有过度的压力用3ml 4℃冷心脏停搏液。避免空气进入冠脉循环。掉落冰冷的生理盐水到移植物，以进一步提高心脏骤停。 </li><li>样近端主动脉至头臂干的起源。 </li><li>结扎下，并均优于腔静脉靠近心脏用8-0丝线和远端横切他们的连字。 </li><li>解剖游离肺动脉干的远端越好。结扎接近分叉8-​​0丝绸肺​​动脉和横切其远端。 </li><li>用一个8-0丝线结扎结扎肺静脉一起，横切它们。 </li><li>从供体取出心脏，并将其存储在冰冷的心脏停搏液，直到植入。 </li></ol> 2.收件人准备<ol><li>备通过切割聚酰亚胺管具有11号解剖刀或在显微镜下剪刀的袖口吻合。袖带包括一个圆柱体和一个延伸部分（圆周的1/3），用于安置所述血管夹，每1毫米（图2b）的长度的。 </li><li>麻醉动物，并确认手术耐受如上所述。注入0.25毫升温生理盐水溶液皮下注射（SC）的补液。 </li><li>卸下右颈部区域的使用的电动剃须刀的头发，然后将动物在一个温暖垫仰卧位置。固定双腿的胶带，同时避免过度拉伸的前肢，因为这会危及呼吸链。封面使用眼药膏，以防止干燥的眼睛。消毒手术部位三次洗必泰。 </li><li>做一个横向皮肤切口从右侧下颌角到颈静脉切迹。 </li><li>说白了动员分机ernal颈静脉。烧灼偏科用双极钳和横切它们。 </li><li>划分结扎的颈外静脉与下颌下腺静脉汇合处的水平。 </li><li>通过袖带拉颈外静脉，并通过将一血管钳上的袖带的延伸堵塞血管近侧。取出结扎和灌溉管腔1:10肝素生理盐水。 </li><li>外翻血管壁在压脉袋和与预设的圆周8-0丝线结扎固定。 </li><li>删除胸锁乳突肌的肤浅的一部分。 </li><li>动员的右颈总动脉和横切下面颈动脉分叉连字之间的容器中。 </li><li>通过袖带拉颈动脉和通过将Yasargil夹具上的袖带的延伸堵塞血管近侧。取出结扎和灌溉管腔1:10肝素生理盐水。 </li><li>轻轻dilatE中的动脉管腔与血管扩张器，外翻血管壁在袖口和预设的圆周8-0丝线结扎固定。 </li><li>为了获得足够的空间用于移植植入，取出颌下腺右叶。 </li><li>保持术野湿润，待注入。 </li></ol> 3.植骨<ol><li>放置在收件人颈部接枝与指向内侧主动脉和肺动脉横向倒置。 </li><li>拉移植物的主动脉在与该颈动脉的压脉袋，并固定吻合圆周8-0丝线结扎。 </li><li>切开肺动脉干在其前方面和在与该外部颈静脉压脉袋拉出。再次确保吻合圆周8-0丝线结扎。 </li><li>除去外部颈静脉夹具，通过松开所述颈动脉以下。心中有血立刻填补。提升自主eperfusion下降37℃温生理盐水溶液涂布到移植物。一般情况下，心脏的发展在一分钟内窦性心律。取出袖口延伸静脉部位。 </li><li>确保正确的移植物的位置，吻合口必须是无张力和船只没有扭转。不要在皮肤，因为这烧灼血管可能影响伤口愈合。出血一般自动停止。关闭皮肤用一个8-0连续缝合。 </li></ol> 4.术后护理<ol><li>注入0.25毫升温暖的生理盐水SC补液。 </li><li>为了对抗毒品注射纳洛酮（1.2毫克/千克），氟马西尼（0.5毫克/千克）和atipamezol（2.5毫克/千克体重）SC左动物的保暖垫，直到它已经恢复足够的意识，以保持胸骨斜卧。观察动物的至少一个附加小时到注册任何异常行为。不返回已经历手术给其他动物的公司，直到完全recove动物红色。 </li><li>提供超前镇痛丁丙诺啡（0.05毫克/千克SC每天三次）或根据本地机构协议72小时的步骤后的最小持续时间的替代镇痛。 </li><li>外科医生和一名兽医应定期看动物。如果体重下降&gt; 15％，冷漠，肿胀或手术侧感染动物的使用终端异氟烷吸入安乐死。 </li></ol>

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K., Dong, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+kidney+transplantation+in+mice:+technique+using+cuff+for+renal+vein+anastomosis.">Orthotopic kidney transplantation in mice: technique using cuff for renal vein anastomosis.</a> PloS one. 8, e77278 (2013).</li><li>Maglione, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+technique+for+heterotopic+vascularized+pancreas+transplantation+in+mice+to+assess+ischemia+reperfusion+injury+and+graft+pancreatitis.">A novel technique for heterotopic vascularized pancreas transplantation in mice to assess ischemia reperfusion injury and graft pancreatitis.</a> Surgery. 141, 682-689 (2007).</li><li>Sucher, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+hind-limb+transplantation+in+rats.">Orthotopic hind-limb transplantation in rats.</a> J Vis Exp : JoVE. , (2010).</li><li>Kamada, N., Calne, R. 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The authors have nothing to disclose.

我们已经完成了超过200个心脏移植，成功率为90％，在各种实验设置。与这里所描述的技术中，完整的程序，可在60分钟内由有经验的外科医生进行。由器官获取之前制备颈部血管，总接枝缺血时间可以限制到20分钟。时间用于植入（热缺血时间）是显著缩短使用袖套法相比，缝合技术16。 而不是简单地切除如前所述5,17的心脏，我们通过灌注移植与临床使用的?...

在心脏移植模型经常用于研究I / R损伤，移植排斥反应，移植后血管病变和免疫调节剂的效率。小鼠模型结合许多优点，如公知的免疫学和遗传背景，许多近交和转基因品系和相对低的成本的实验的可用性。 1973年，腹部心脏移植小鼠的技术由科里等 [1]首次描述。在此模型中的移植物是由升主动脉到主肺动脉到下腔静脉的收信人的腹主动脉和的端 - 侧吻合术吻合血管。 1991年，宫颈心脏移植小鼠缝合技术由陈等人 2被描述。在此模型中一个逆行冠状动脉灌注由收件人的颈动脉的吻合建立到克的升主动脉船尾。经由冠状窦静脉血液排入右心房，右心室，并通过肺动脉到受体颈外静脉（图1）排出。相较于腹腔移植植入颈椎模型是创伤小，移植物的位置表浅能够很容易获得额外的随访检查（ 如触诊，超声心动图，活体荧光显微镜）3,4。 尽管技术改进5,6，更广泛地使用这种模式已被有限的，由于技术上的困难进行了小血管缝合吻合术吻合口瘘和血栓形成的高发期。 1991年，松浦晃一郎等人 。引入其中血管壁被外翻在一个合成柱面，大大方便了颈部心脏移植7的模型的袖口技术。该技术是福rther通过了各种实验移植模型，包括8肾，胰9，四肢10，11,12肝，肺13,14和血管移植15。相比于缝合技术吻合术的所需时间，因此，热缺血时间较短，有显著较少血管并发症使用袖套法16。此外，它使医生与显微稍加培训与成功率高执行该操作。袖带技术的缺点是在颈总动脉，这可能会改变脑灌注的前提结扎。 在这个视频文章中，我们介绍了宫颈心脏移植小鼠改进和简化套管法。

<table><tbody><tr><td>Forceps JFC-7.2</td><td>S&amp;amp;T</td><td>P-00036</td><td>Curved tip 0.20 mm</td></tr><tr><td>Vessel dilatator D-5a</td><td>S&amp;amp;T</td><td>S-00124</td><td></td></tr><tr><td>Adventita scissor</td><td>S&amp;amp;T</td><td>S-00102</td><td></td></tr><tr><td>Vascular clamp B-1V</td><td>S&amp;amp;T</td><td>S-00396</td><td></td></tr><tr><td>Yasargil Clip</td><td>Peter Lazic</td><td>65,097</td><td>3.5 mm lenght</td></tr><tr><td>Bipolar forceps</td><td>Micromed</td><td>148-100-011</td><td>tip 0.25 mm</td></tr><tr><td>Polyimide tubes</td><td>River Tech Medical</td><td></td><td>19-24 gauge</td></tr><tr><td>8.0 silk ligatures</td><td>Catgut</td><td></td><td></td></tr><tr><td>Custodiol HTK solution</td><td>Essential Pharmaceuticals</td><td></td><td></td></tr></tbody></table>

heterotopic cervical heart transplantation in mice

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies this model by avoiding challenging suture anastomoses of small vessels thereby reducing warm ischemia time. In comparison to abdominal graft implantation the cervical model is less invasive and the implanted graft is easily accessible for further follow-up examinations. Anastomoses are performed by pulling the ascending aorta of the graft over the cuff with the recipient&#8217;s common carotid artery and by pulling the main pulmonary artery over the cuff with the external jugular vein. Selection of appropriate cuff size and complete mobilization of the vessels are important for successful revascularization. Ischemia-reperfusion (I/R) injury can be minimized by perfusing the graft with a cardioplegic solution and by hypothermia. In this article, we provide technical details for a simplified and improved cuff technique, which should allow surgeons with basic microsurgical skills to perform the procedure with a high success rate.

我们的团队经过大约20个训练手术能成功执行所描述的技术。最具挑战性的部分是外翻颈动脉上的压脉袋的血管壁，因为它很容易翻转回来，多次尝试可能导致撕裂壁。训练期间之后，平均总手术时间为60±8分钟。大约20分钟都需要捐助的收获，25分钟准备的颈部血管和15分钟吻合及皮肤缝合的。 心肌I / R损伤的调查，我们进行了200的操作在同系的C57BL / 6小鼠中各种实验设置与92...

Watch this Scientific Journal Video about Heterotopic Cervical Heart Transplantation in Mice at JoVE.com

Heterotopic Cervical Heart Transplantation in Mice

The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.

颈椎异位心脏移植小鼠

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

heterotopic-cervical-heart-transplantation-in-mice

Research

JoVE Journal

Medicine

11.1K 次观看.  German Heart Center Munich, Technische Universität München. The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.

视频: Heterotopic Cervical Heart Transplantation in Mice

Heterotopic Heart Transplantation in Mice

The mouse heterotopic heart transplantation has been used widely since it was introduced by Drs. Corry and Russell in 1973. It is particularly valuable for studying rejection and immune response now that newer transgenic and gene knockout mice are available, and a large number of immunologic reagents have been developed. The heart transplant model is less stringent than the skin transplant models, although technically more challenging. We have developed a modified technique and have completed over 1000 successful cases of heterotopic heart transplantation in mice. When making anastomosis of the ascending aorta and abdominal aorta, two stay sutures are placed at the proximal and distal apexes of recipient abdominal aorta with the donor s ascending aorta, then using 11-0 suture for anastomosis on both side of aorta with continuing sutures. The stay sutures make the anastomosis easier and 11-0 is an ideal suture size to avoid bleeding and thrombosis.
When making anastomosis of pulmonary artery and inferior vena cava, two stay sutures are made at the proximal apex and distal apex of the recipient s inferior vena cava with the donor s pulmonary artery. The left wall of the inferior vena cava and donor s pulmonary artery is closed with continuing sutures in the inside of the inferior vena cava after, one knot with the proximal apex stay suture the right wall of the inferior vena cava and the donor s pulmonary artery are closed with continuing sutures outside the inferior vena cave with 10-0 sutures. This method is easier to perform because anastomosis is made just on the one side of the inferior vena cava and 10-0 sutures is the right size to avoid bleeding and thrombosis. In this article, we provide details of the technique to supplement the video.

The mouse heterotopic heart transplantation model has been proven by many investigators to be an important method for studying mechanisms of rejection and immune response. However, the techniques involved are still challenging. By modifying standard techniques we have had success with more than 1000 transplants.

小鼠异位心脏移植

The mouse heterotopic heart transplantation has been used widely since it was introduced by Drs. Corry and Russell in 1973. It is particularly valuable ...

科研

生物学

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are available to perform mechanistic in vivo studies. While heart transplantation models using a suture technique were first successfully developed in rats, the translation into an equally widespread used murine equivalent was never achieved due the technical complexity of the microsurgical procedure. In contrast, non-suture cuff techniques, also developed initially in rats, were successfully adapted for use in mice1-3. This technique for revascularization involves two major steps I) everting the recipient vessel over a polyethylene cuff; II) pulling the donor vessel over the formerly everted recipient vessel and holding it in place with a circumferential tie. This ensures a continuity of the endothelial layer, short operating time and very high patency rates4.
Using this technique for vascular anastomosis we performed more than 1,000 cervical heart transplants with an overall success rate of 95%. For arterial inflow the common carotid artery and the proximal aortic arch were anastomosed resulting in a retrograde perfusion of the transplanted heart. For venous drainage the pulmonary artery of the graft was anastomosed with the external jugular vein of the recipient5.
Herein, we provide additional details of this technique to supplement the video.

The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach.
Herein, we provide additional details of this technique to supplement the video.

用改进的袖套法小鼠颈部心脏移植模型

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

医学

A Modified Method for Heterotopic Mouse Heart Transplantion

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents available. A difficulty is presented due to the small size of the animal and the considerable technical challenges of the microsurgery involved in heart transplantation. In particular, a high rate of technical failure early after transplantation may result from recipient death and post-operative complications such as hind limb paralysis or a non-beating heart. Here, the complete technique for heterotopic mouse heart transplantation is demonstrated, involving harvesting the donor heart and its subsequent implantation into a recipient mouse. The donor heart is harvested immediately following in situ perfusion with cold heparinized saline and transection of the ascending aorta and pulmonary artery. The recipient operation involves preparation of the abdominal aorta and inferior vena cava (IVC), followed by end-to-side anastomosis of the donor aorta with the recipient aorta using a single running 10-0 microsuture and a similar anastomosis of the donor pulmonary artery with the recipient IVC. Following the operation the animal is injected with 0.6 ml normal saline subcutaneously and allowed to recover on a 37&#160;&#176;C heating pad. The results from 227 mouse heart transplants are summarized with a success rate at 48 hr of 86.8%. Of the 13.2% failures within 48 hr, 5 (2.2%) experienced hind limb paralysis, 10 (4.4%) had a non-beating heart due to graft ischemic injury and/or thrombosis, while 15 (6.6%) died within 48 hr.

A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.

对于异位小鼠心脏移植技术的一种改进方法

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents ...

Murine Heterotopic Heart Transplant Technique

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become proficient in and utilize this technique, it is now widely used by many laboratories around the world. A significant refinement to the original technique was developed and reported in 2001 by Niimi. Described here are the techniques that have evolved over more than a decade in the hands of three surgeons (Plenter, Grazia, Pietra) in our center. These techniques are now being passed on to a younger generation of surgeons and researchers.
Based largely on the Niimi experience, the procedures used have evolved in the fine details - details which we will endeavor to relate here in such a way that others may be able to use this very useful model. Like Niimi, we have found that a video aid to learning is a priceless resource for the beginner.

The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.

小鼠异位心脏移植技术

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice

Exploration of novel strategies in organ transplantation to prolong allograft survival and minimizing the need for long-term maintenance immunosuppression must be pursued. Employing vascularized bone marrow transplantation and co-transplantation of the thymus have shown promise in this regard in various animal models.1-11 Vascularized bone marrow transplantation allows for the uninterrupted transfer of donor bone marrow cells within the preserved donor microenvironment, and the incorporation of thymus tissue with vascularized bone marrow transplantation has shown to increase T-cell chimerism ultimately playing a supportive role in the induction of immune regulation. The combination of solid organ and vascularized composite allotransplantation can uniquely combine these strategies in the form of a novel transplant model. Murine models serve as an excellent paradigm to explore the mechanisms of acute and chronic rejection, chimerism, and tolerance induction, thus providing the foundation to propagate superior allograft survival strategies for larger animal models and future clinical application. Herein, we developed a novel heterotopic en bloc chest wall, thymus, and heart transplant model in mice using a cervical non-suture cuff technique. The experience in syngeneic and allogeneic transplant settings is described for future broader immunological investigations via an instructional manuscript and video supplement.

To study combined solid organ and vascularized composite allotransplantation, we describe a novel heterotopic en bloc chest wall, thymus, and heart transplant model in mice using a cervical non-suture cuff technique.

一种新的显微模型异位，整块胸壁，胸腺，和心脏移植小鼠

Exploration of novel strategies in organ transplantation to prolong allograft survival and minimizing the need for long-term maintenance immunosuppression ...

An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats

Heterotopic heart transplantation in rats has been a commonly used model for diverse immunological studies for more than 50 years. Several modifications have been reported since the first description in 1964. After 30 years of performing heterotopic heart transplantation in rats, we have developed a simplified surgical approach, which can be easily taught and performed without further surgical training or background.
After dissection of the ascending aorta and the pulmonary artery and ligation of superior and inferior caval and pulmonary veins, the donor heart is harvested and subsequently perfused with ice-cold saline solution supplemented with heparin. After clamping and incising the recipient abdominal vessels, the donor ascending aorta and pulmonary artery are anastomosed to the recipient abdominal aorta and inferior vena cava, respectively, using continuous running sutures.
Depending on different donor-recipient combinations, this model allows analyses of either acute or chronic rejection of allografts. The immunological significance of this model is further enhanced by a novel approach of in-ear injection of vital cardiac muscle cells and subsequent analysis of draining cervical lymphatic tissue.

We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats.

大鼠异位心脏和心肌细胞移植的免疫模型

Heterotopic heart transplantation in rats has been a commonly used model for diverse immunological studies for more than 50 years. Several modifications ...

免疫与感染

Optimization of the Cuff Technique for Murine Heart Transplantation

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to improve surgical efficiency. In our lab, we have optimized the cuff technique with two major steps. First, we used the inner tube technique to insert a temporary inner tube into the external jugular vein and carotid artery blood vessel to facilitate eversion of the vessel over the cuff. Second, we performed complete heterotopic cardiac transplantation through the collaboration of two experienced surgeons. These modifications effectively reduced the operation time to 25 minutes, with a success rate of 95%. In this report, we describe these procedures in detail and provide a supplemental video. We believe that this report on the improved cuff technique will offer practical guidance for murine heterotopic heart transplantation and will enhance the utility of this mouse model for basic research.

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

穆林心脏移植袖口技术的优化

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart failure. Multiple small-animal models of heart transplantation have been used to study the acute and long-term effects of novel therapies. However, few are tested and demonstrated success in clinical trials. It is of critical importance to evaluate new therapies in a clinically relevant large-animal model for efficient and reliable translation of basic studies&#39;&#160;findings. Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and previously used to investigate novel cardioprotective strategies. This procedure focuses on acute ischemia-reperfusion injury and is a reliable method to investigate novel interventions which have been tested and validated in smaller experimental models, such as the murine model. We demonstrate its usefulness in assessing cardiac performance during the early post-transplantation period and other potential possibilities enabled by the model.

Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and utilized to investigate novel cardioprotective strategies.

正交心脏移植的临床前猪模型

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after transplantation, and the impact of immunomodulatory therapies on graft rejection. Heterotopic cervical heart transplantation in mice was first described in 1991 using sutured anastomoses and subsequently modified to include cuff techniques. This modification allowed for improved success rates, and since then, there have been multiple reports that have proposed further technical improvements. However, translation into more widespread utilization remains limited due to the technical difficulty associated with graft anastomoses, which requires precision to achieve adequate length and caliber of the cuffs to avoid vascular anastomotic twisting or excessive tension, which can result in damage to the graft. The present protocol describes a modified technique for performing heterotopic cervical cardiac transplantation in mice which involves cuff placement on the recipient's common carotid artery and the donor's pulmonary artery in alignment with the direction of the blood flow.

Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.

利用血管袖带的小鼠异位宫颈心脏移植

Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after ...

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

The surgical technique of heterotopic abdominal heart transplantation in mice is a standard model for research in transplantation immunology. Here, the established technique for a modified blood circuit reconstruction in a heterotopic abdominal heart transplantation model is presented. This method uses the intrathoracic inferior vena cava (IIVC) instead of the pulmonary artery of the donor heart for the anastomosis to the inferior vena cava of the recipient. It is facilitating and improving success rates for abdominal heart transplantation in mice.

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

腹部小鼠心脏移植模型中的血液回路重建

The surgical technique of heterotopic abdominal heart transplantation in mice is a standard model for research in transplantation immunology. Here, the ...