Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal NeuronsMaría Calvo-Rodríguez 1, Carlos Villalobos 1, Lucía Nuñez 2
1Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid
Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.
