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Consejo Superior de Investigaciones Cientìficas

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Fluorescence and Bioluminescence Imaging of Subcellular Ca²⁺ in Aged Hippocampal Neurons

María Calvo-Rodríguez¹, Carlos Villalobos¹, Lucía Nuñez²

¹Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, ²Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid

Intracellular Ca²⁺ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca²⁺ overload. We aimed at investigating Ca²⁺ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca²⁺ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.