National Chiayi University
2 ARTICLES PUBLISHED IN JoVE
Biology
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
Kun-Wei Chan 1, Chieh Lo 1, Chi-Shih Chu 2, Li-Te Chin 2, Yu-Ting Wang 1, Wei-Cheng Yang 1
1Department of Veterinary Medicine, National Chiayi University, 2Department of Microbiology and Immunology/Graduate Institute of Biomedical and Biopharmaceutical Sciences, National Chiayi University
This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.
Environment
Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
Wen-Ta Li 1, Bang-Yeh Liou 1, Wei-Cheng Yang 2, Meng-Hsien Chen 3, Hui-Wen Chang 1,2, Hue-Ying Chiou 4, Victor Fei Pang 1,2, Chian-Ren Jeng 1,2
1Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, 2School of Veterinary Medicine, National Taiwan University, 3Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 4Graduate Institute of Veterinary Pathobiology, National Chung Hsing University
A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.
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