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National Chiayi University

2 ARTICLES PUBLISHED IN JoVE

Biology

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Kun-Wei Chan¹, Chieh Lo¹, Chi-Shih Chu², Li-Te Chin², Yu-Ting Wang¹, Wei-Cheng Yang¹

¹Department of Veterinary Medicine, National Chiayi University, ²Department of Microbiology and Immunology/Graduate Institute of Biomedical and Biopharmaceutical Sciences, National Chiayi University

This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.

Environment

Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

Wen-Ta Li¹, Bang-Yeh Liou¹, Wei-Cheng Yang², Meng-Hsien Chen³, Hui-Wen Chang^1,2, Hue-Ying Chiou⁴, Victor Fei Pang^1,2, Chian-Ren Jeng^1,2

¹Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, ²School of Veterinary Medicine, National Taiwan University, ³Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, ⁴Graduate Institute of Veterinary Pathobiology, National Chung Hsing University

A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.