Wake Forest Univeristy Health Sciences
2 ARTICLES PUBLISHED IN JoVE
Medicine
Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
Sean V. Murphy 1, Amritha Kidyoor 1, Tanya Reid 1, Anthony Atala 1, Euan M. Wallace 2, Rebecca Lim 2
1Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, 2The Ritchie Centre, Monash Institute of Medical Research, Monash University
We describe a protocol to isolate and culture human amnion epithelial cells (hAECs) using animal product-free reagents in accordance with current good manufacturing practices (cGMP) guidelines.
Bioengineering
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
Aleksander Skardal 1, Mahesh Devarasetty 1, Hyun-Wook Kang 1, Young-Joon Seol 1, Steven D. Forsythe 1, Colin Bishop 1, Thomas Shupe 1, Shay Soker 1, Anthony Atala 1
1Wake Forest Institute for Regenerative Medicine, Wake Forest Univeristy Health Sciences
We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.
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