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Wake Forest Univeristy Health Sciences

2 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Sean V. Murphy¹, Amritha Kidyoor¹, Tanya Reid¹, Anthony Atala¹, Euan M. Wallace², Rebecca Lim²

¹Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, ²The Ritchie Centre, Monash Institute of Medical Research, Monash University

We describe a protocol to isolate and culture human amnion epithelial cells (hAECs) using animal product-free reagents in accordance with current good manufacturing practices (cGMP) guidelines.

Bioengineering

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Aleksander Skardal¹, Mahesh Devarasetty¹, Hyun-Wook Kang¹, Young-Joon Seol¹, Steven D. Forsythe¹, Colin Bishop¹, Thomas Shupe¹, Shay Soker¹, Anthony Atala¹

¹Wake Forest Institute for Regenerative Medicine, Wake Forest Univeristy Health Sciences

We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.