University of Quebec in Montreal

1 ARTICLES PUBLISHED IN JoVE

Genetics

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Adjimon Gatien Lokossou¹, Chirine Toufaily², Amandine Vargas³, Benoit Barbeau¹

¹Department of Biological Sciences, University of Quebec in Montreal, ²Department of Pharmacology and Therapeutics, McGill University, ³Department of Clinical Sciences, University of Montreal

This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.