siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous CytotrophoblastsAdjimon Gatien Lokossou 1, Chirine Toufaily 2, Amandine Vargas 3, Benoit Barbeau 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal
This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.
