Virginia Maryland College of Veterinary Medicine, Virginia Tech
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
Irving C. Allen 1
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University
The host immune response to pathogen infection is a tightly regulated process. Utilizing a lipopolysaccharide lung exposure model in mice, it is possible to conduct high resolution evaluations of the complex mechanisms associated with disease pathogenesis.
Immunology and Infection
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
Daniel E. Rothschild 1, Tara Srinivasan 2, Linette A. Aponte-Santiago 1, Xiling Shen 2,3,4, Irving C. Allen 1
1Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering, Cornell University, 3School of Electrical and Computer Engineering, Cornell University, 4Department of Biomedical Engineering, Duke University
Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.
