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Virginia Maryland College of Veterinary Medicine, Virginia Tech

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice

Irving C. Allen¹

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University

The host immune response to pathogen infection is a tightly regulated process. Utilizing a lipopolysaccharide lung exposure model in mice, it is possible to conduct high resolution evaluations of the complex mechanisms associated with disease pathogenesis.

Immunology and Infection

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Daniel E. Rothschild¹, Tara Srinivasan², Linette A. Aponte-Santiago¹, Xiling Shen^2,3,4, Irving C. Allen¹

¹Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, ²Department of Biomedical Engineering, Cornell University, ³School of Electrical and Computer Engineering, Cornell University, ⁴Department of Biomedical Engineering, Duke University

Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.