The Jerzy Kukuczka Academy of Physical Education
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery
Ana R. Leda 1, Levy Dygert 1, Luc Bertrand 1, Michal Toborek 1,2
1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2The Jerzy Kukuczka Academy of Physical Education
The present protocol describes a mouse microsurgery infusion technique, which effectively delivers substances directly into the brain via the internal carotid artery.
Medicine
Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
Luc Bertrand 1,2, Levi Dygert 2, Michal Toborek 1,3
1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2Miller School of Medicine, University of Miami, 3Jerzy Kukuczka Academy of Physical Education
We describe a mouse model of stroke induced by the occlusion of the middle cerebral artery using a silicone coated suture. The protocol can be applied to induce permanent occlusion or a temporary ischemia, followed by reperfusion.
Biochemistry
A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
Nikolai Fattakhov 1, Silvia Torices 1, Sarah Becker 1, Timea Teglas 1, Oandy Naranjo 1, Michal Toborek 1,2
1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2Institute of Physiotherapy and Health Sciences, The Jerzy Kukuczka Academy of Physical Education
Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates.
