Düsseldorf University Hospital
2 ARTICLES PUBLISHED IN JoVE
Medicine
Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video
Zuzanna Rowinska 1,2, Simone Gorressen 3, Marc W. Merx 4, Thomas A. Koeppel 5, Alma Zernecke 6, Elisa A. Liehn 2
1Department of Vascular Surgery and Interdisciplinary Vein Center, St. Josef-Hospital, Ruhr- University Bochum, 2Institute of Molecular Cardiovascular Research, University Hospital, RWTH Aachen University Department of Medicine, 3Institute of Pharmacology and Clinical Pharmacology, Düsseldorf University Hospital, 4Department of Cardiology, Vascular Medicine and Intensive Care Medicine, Hospital Robert Koch Gehrden, 5Division of Vascular Surgery, Asklepios Klinik St. Georg, 6Institute of Experimental Biomedicine, Würzburg University
We present an orthotopic aortic transplantation model using the sleeve technique in mice. It is a very rapid anastomosis method, which can be employed in studies of vascular disease.
Neuroscience
Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
Rodrigo Lerchundi *1, Karl W. Kafitz *1, Marcel Färfers 1, Felix Beyer 2, Na Huang 1, Christine R. Rose 1
1Institute of Neurobiology, Heinrich Heine University Düsseldorf, 2Department of Neurology, Düsseldorf University Hospital
We describe a protocol for cell-type specific expression of the genetically encoded FRET-based sensor ATeam1.03YEMK in organotypic slice cultures of the mouse forebrain. Furthermore, we show how to use this sensor for dynamic imaging of cellular ATP levels in neurons and astrocytes.
