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Düsseldorf University Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

Zuzanna Rowinska^1,2, Simone Gorressen³, Marc W. Merx⁴, Thomas A. Koeppel⁵, Alma Zernecke⁶, Elisa A. Liehn²

¹Department of Vascular Surgery and Interdisciplinary Vein Center, St. Josef-Hospital, Ruhr- University Bochum, ²Institute of Molecular Cardiovascular Research, University Hospital, RWTH Aachen University Department of Medicine, ³Institute of Pharmacology and Clinical Pharmacology, Düsseldorf University Hospital, ⁴Department of Cardiology, Vascular Medicine and Intensive Care Medicine, Hospital Robert Koch Gehrden, ⁵Division of Vascular Surgery, Asklepios Klinik St. Georg, ⁶Institute of Experimental Biomedicine, Würzburg University

We present an orthotopic aortic transplantation model using the sleeve technique in mice. It is a very rapid anastomosis method, which can be employed in studies of vascular disease.

Neuroscience

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03^YEMK

Rodrigo Lerchundi^*1, Karl W. Kafitz^*1, Marcel Färfers¹, Felix Beyer², Na Huang¹, Christine R. Rose¹

¹Institute of Neurobiology, Heinrich Heine University Düsseldorf, ²Department of Neurology, Düsseldorf University Hospital

We describe a protocol for cell-type specific expression of the genetically encoded FRET-based sensor ATeam1.03^YEMK in organotypic slice cultures of the mouse forebrain. Furthermore, we show how to use this sensor for dynamic imaging of cellular ATP levels in neurons and astrocytes.