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Health Sciences Centre

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Low-Density Primary Hippocampal Neuron Culture

Reiko T. Roppongi^*1,2, Kevin P. Champagne-Jorgensen^*1,2, Tabrez J. Siddiqui^1,2

¹Department of Physiology and Pathophysiology, University of Manitoba, ²Kleysen Institute for Advanced Medicine, Health Sciences Centre

This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.

Neuroscience

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

Noriko Koganezawa¹, Reiko T. Roppongi², Yuko Sekino^3,4, Izuo Tsutsui³, Ayaka Higa⁵, Tomoaki Shirao^1,5

¹Department of Pharmacology, Graduate School of Medicine, Gunma University, ²Gunma University Initiative for Advanced Research, Gunma University, ³Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, ⁴Institute for Drug Discovery Innovation, ⁵AlzMed, Inc.

A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate.