Miller School of Medicine, University of Miami
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
Natasa Strbo 1, Laura Romero 1, Maria Alcaide 2, Margaret Fischl 2
1Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, 2Division of Infectious Diseases, Miller School of Medicine, University of Miami
We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.
Immunology and Infection
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
Petoria Gayle 1, Nancy E. Freitag 2, Natasa Strbo 1, Kurt Schesser 1
1Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 2Department of Microbiology & Immunology, University of Illinois College of Medicine
We describe both in vitro and in vivo infection assays that can be used to analyze the activities of host-encoding factors.
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