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Miller School of Medicine, University of Miami

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

Natasa Strbo¹, Laura Romero¹, Maria Alcaide², Margaret Fischl²

¹Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, ²Division of Infectious Diseases, Miller School of Medicine, University of Miami

We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.

Immunology and Infection

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Petoria Gayle¹, Nancy E. Freitag², Natasa Strbo¹, Kurt Schesser¹

¹Department of Microbiology & Immunology, University of Miami Miller School of Medicine, ²Department of Microbiology & Immunology, University of Illinois College of Medicine

We describe both in vitro and in vivo infection assays that can be used to analyze the activities of host-encoding factors.