Parkinson’s Institute and Clinical Center
2 ARTICLES PUBLISHED IN JoVE
Medicine
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Malini Vangipuram 1, Dennis Ting 1, Sam Kim 1, Robert Diaz 1, Birgitt Schüle 1
1Basic Research Department, The Parkinson's Institute
The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.
Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
Kelsey Lee *1, Ou Chong Saetern *1, Andrew Nguyen 1, Leonardo Rodriguez 1, Birgitt Schüle 1
1Parkinson’s Institute and Clinical Center
The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.
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