Accedi

Parkinson’s Institute and Clinical Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

Malini Vangipuram¹, Dennis Ting¹, Sam Kim¹, Robert Diaz¹, Birgitt Schüle¹

¹Basic Research Department, The Parkinson's Institute

The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.

Neuroscience

Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors

Kelsey Lee^*1, Ou Chong Saetern^*1, Andrew Nguyen¹, Leonardo Rodriguez¹, Birgitt Schüle¹

¹Parkinson’s Institute and Clinical Center

The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.