Sotera Defense Solutions
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
Okhil K. Nag 1, Jawad Naciri 1, Eunkeu Oh 2,3, Christopher M. Spillmann 1, James B. Delehanty 1
1Center for Biomolecular Science and Engineering, Naval Research Laboratory, 2Optical Sciences Division, Naval Research Laboratory, 3Sotera Defense Solutions
A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living cells.
Biology
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
Bruce Nguyen Tran 1, Eunkeu Oh 2, Kimihiro Susumu 2,3, Mason Wolak 2, Kirill Gorshkov 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation
In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.
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