Accedi

Sotera Defense Solutions

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Okhil K. Nag¹, Jawad Naciri¹, Eunkeu Oh^2,3, Christopher M. Spillmann¹, James B. Delehanty¹

¹Center for Biomolecular Science and Engineering, Naval Research Laboratory, ²Optical Sciences Division, Naval Research Laboratory, ³Sotera Defense Solutions

A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living cells.

Biology

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Bruce Nguyen Tran¹, Eunkeu Oh², Kimihiro Susumu^2,3, Mason Wolak², Kirill Gorshkov¹

¹National Center for Advancing Translational Sciences, ²Optical Sciences Division, Code 5600, Naval Research Laboratory, ³Jacobs Corporation

In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.