Baylor College of Medicine, Texas Children's Hospital
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
Thomas Lee 1, Ivan Shevchenko 1, Maran L. Sprouse 1, Maria Bettini 1, Matthew L. Bettini 1
1Department of Pediatrics, Baylor College of Medicine
We present a rapid and flexible protocol for a single T cell receptor (TCR) retroviral-based in vivo expression system. Retroviral vectors are used to transduce bone marrow progenitor cells to study T cell development and function of a single TCR in vivo as an alternative to TCR transgenic mice.
Immunology and Infection
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
Maran L. Sprouse 1, Gabriele Blahnik 2, Thomas Lee 1, Natalie Tully 1, Pinaki Benarjee 3, Eddie A. James 2, Maria J. Redondo 4, Matthew L. Bettini 1, Maria Bettini 1
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital
The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
