Yamagata University

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Izumi Nishidate¹, Afrina Mustari¹, Satoko Kawauchi², Shunichi Sato², Manabu Sato³

¹Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology, ²Division of Biomedical Information Sciences, National Defense Medical College Research Institute, ³Graduate School of Science and Engineering, Yamagata University

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system.

Bioengineering

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Afrina Mustari^*1, Izumi Nishidate^*1, Md. Abdul Wares^1,6, Takaaki Maeda², Satoko Kawauchi³, Shunichi Sato³, Manabu Sato⁴, Yoshihisa Aizu⁵

¹Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology, ²Department of Mechanical Engineering, Kushiro National College of Technology, ³Division of Bioinformation and Therapeutic Systems, National Defense Medical College Research Institute, ⁴Graduate School of Science and Engineering, Yamagata University, ⁵College of Design and Manufacturing Technology, Muroran Institute of Technology, ⁶Department of Livestock Services, Ministry of Fisheries and Livestock, Government of Bangladesh

Here, we demonstrate how agarose-based tissue-mimicking optical phantoms are made and how their optical properties are determined using a conventional optical system with an integrating sphere.

Genetics

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Hirofumi Nishizono^*1,2,3, Mohamed Darwish^*4,5, Hideki Uosaki^6,7, Nanami Masuyama^8,9,10, Motoaki Seki^8,11, Hiroyuki Abe³, Nozomu Yachie^8,9,10,12,13, Ryohei Yasuda¹

¹Max Planck Florida Institute for Neuroscience, ²Life Science Research Center, University of Toyama, ³Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, ⁴Graduate School of Innovative Life Science, University of Toyama, ⁵Department of Biochemistry, Faculty of Pharmacy, Cairo University, ⁶Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, ⁷Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, ⁸Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, ⁹Institute for Advanced Biosciences, Keio University, ¹⁰Graduate School of Media and Governance, Keio University, ¹¹Department of Molecular Oncology, Graduate School of Medicine, Chiba University, ¹²Department of Biological Sciences, School of Science, University of Tokyo, ¹³College of Arts and Sciences, University of Tokyo

Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.