Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
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CRISPR Electroporation
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Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
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Conclusion
副本
Our protocol facilitates the preparation of genetically modified mice easily, efficiently, and rapidly from single cell mouse embryos. Our protocol combine the use of freeze-thawed one-cell stage embryos and electroporation allowing the rapid gene
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Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.