Sherbrooke Institute of Pharmacology
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat
Maggie Roy 1, Scott Nugent 1, Sébastien Tremblay 2, Maxime Descoteaux 3, Jean-François Beaudoin 2, Luc Tremblay 2, Roger Lecomte 2,4, Stephen C Cunnane 1
1Research Center on Aging and Department of Physiology and Biophysics, Université de Sherbrooke, 2Sherbrooke Molecular Imaging Center, Étienne-Le Bel Clinical Research Center, Université de Sherbrooke, 3Department of Computer Science, Université de Sherbrooke, 4Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke
Small-animal positron emission tomography enables the assessment of the brain's two main energy substrates: glucose and ketones. In the present method, 11C-acetoacetate and 18F-fluorodeoxyglucose are injected sequentially in each animal, and their uptake is measured quantitatively in specific brain regions determined from the magnetic resonance images.
Bioengineering
Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
Simon Beaudoin 1, Michel Paquette 1, Laurent Fafard-Couture 1, Mylene A. Tremblay 1, Roger Lecomte 1,2,3, Brigitte Guérin 1,2,3, Jeffrey V. Leyton 1,2,3
1Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke, 2Sherbrooke Molecular Imaging Center (CIMS), Université de Sherbrooke, 3Sherbrooke Institute of Pharmacology
Viral-derived peptides coupled to antibody-conjugates (ACs) is an approach gaining momentum due to the potential of delivering molecular payloads with increased tumor cell accumulation. Utilizing common methods to evaluate peptide conjugation, AC and payload intracellular accumulation, and tumor targeting, this protocol helps researchers during the key initial development phases.
