Sherbrooke Institute of Pharmacology

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat

Maggie Roy¹, Scott Nugent¹, Sébastien Tremblay², Maxime Descoteaux³, Jean-François Beaudoin², Luc Tremblay², Roger Lecomte^2,4, Stephen C Cunnane¹

¹Research Center on Aging and Department of Physiology and Biophysics, Université de Sherbrooke, ²Sherbrooke Molecular Imaging Center, Étienne-Le Bel Clinical Research Center, Université de Sherbrooke, ³Department of Computer Science, Université de Sherbrooke, ⁴Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke

Small-animal positron emission tomography enables the assessment of the brain's two main energy substrates: glucose and ketones. In the present method, ¹¹C-acetoacetate and ¹⁸F-fluorodeoxyglucose are injected sequentially in each animal, and their uptake is measured quantitatively in specific brain regions determined from the magnetic resonance images.

Bioengineering

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Simon Beaudoin¹, Michel Paquette¹, Laurent Fafard-Couture¹, Mylene A. Tremblay¹, Roger Lecomte^1,2,3, Brigitte Guérin^1,2,3, Jeffrey V. Leyton^1,2,3

¹Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke, ²Sherbrooke Molecular Imaging Center (CIMS), Université de Sherbrooke, ³Sherbrooke Institute of Pharmacology

Viral-derived peptides coupled to antibody-conjugates (ACs) is an approach gaining momentum due to the potential of delivering molecular payloads with increased tumor cell accumulation. Utilizing common methods to evaluate peptide conjugation, AC and payload intracellular accumulation, and tumor targeting, this protocol helps researchers during the key initial development phases.