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Rutgers

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Jansy P. Sarathy¹, Hsin-pin Ho Liang¹, Danielle Weiner², Jacqueline Gonzales², Laura E. Via², Véronique Dartois¹

¹Public Health Research Institute Centre, New Jersey Medical School, Rutgers, ²Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH

Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

JoVE Core

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)

Matthew Zimmerman¹, Landry Blanc¹, Pei-Yu Chen¹, Véronique Dartois¹, Brendan Prideaux¹

¹Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey

Here, we describe a protocol using laser capture microdissection coupled with LC/MS analysis to spatially-quantify drug distributions within pulmonary tuberculosis granulomas. The approach has broad applicability to quantifying drug concentrations within tissues at high spatial detail.