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National Institute of Biological Sciences, Beijing

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation
Zhe Liu 1,2, Siling Fu 2,3, Nan Tang 2
1Department of Life Sciences, Peking University, 2National Institute of Biological Sciences, Beijing, 3Graduate School of Peking Union Medical College

Internal lung surface area (ISA) is a critical criterion for assessing lung morphology and physiology in lung diseases and injury-induced alveolar regeneration. We describe here a standardized method that can minimize the measurement bias for ISA in both lung pneumonectomy and prosthesis implantation mouse models.

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Neuroscience

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
Long Mei 1,2, Cheng Zhan 2, Eric Erquan Zhang 2
1PTN Joint Graduate Program, School of Life Sciences, Peking University, 2National Institute of Biological Sciences, Beijing

This newly developed fluorescence-based technology enables long-term monitoring of the transcription of circadian clock genes in the suprachiasmatic nucleus (SCN) of freely moving mice in real-time and at a high temporal resolution.

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Biology

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
Zhaodi Jiang 1,2
1National Institute of Biological Sciences, Beijing, 2Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University

The present protocol describes a clonable electron microscopy labeling technology for detecting metallothionein-tagged proteins in cells using a novel autonucleation suppression mechanism-based gold nanoparticle synthesis technique.

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