Hopital Européen Georges Pompidou

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1⁺ Tim-3⁺ CD8⁺ T Cells

Clémence Granier¹, Emeline Vinatier^2,3,4, Elia Colin^2,3, Marion Mandavit^2,3, Charles Dariane^2,3,5, Virginie Verkarre⁶, Lucie Biard⁷, Rami El Zein², Corinne Lesaffre², Isabelle Galy-Fauroux², Hélène Roussel^2,3,6, Eléonore De Guillebon⁸, Charlotte Blanc^2,3, Antonin Saldmann⁴, Cécile Badoual^2,6, Alain Gey^*4, Éric Tartour^*2,4

¹PARCC-INSERM U970, Université Paris Descartes, ²INSERM U970, Université Paris Descartes, ³Equipe Labellisée Ligue Contre le Cancer, ⁴Department of Immunology, Hopital Européen Georges Pompidou, ⁵Department of Medical Urology, Hopital Européen Georges Pompidou, ⁶Department of Pathology, Hopital Européen Georges Pompidou, ⁷Université Paris Diderot Paris 7, ⁸Department of medical oncology, Hopital Européen Georges Pompidou

Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8⁺T cells. This reproducible and reliable technique is suitable for large cohort analyses.

Genetics

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Sophie Outh-Gauer¹, Jérémy Augustin¹, Marion Mandavit², Ophélie Grard², Thomas Denize¹, Marine Nervo¹, Charles Lépine¹, Marc Rassy², Eric Tartour^2,3, Cécile Badoual^1,2

¹Department of Pathology, Georges Pompidou European Hospital, Assistance publique - Hôpitaux de Paris (APHP), Paris Descartes University, ²Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, ³Laboratory of Immunology, Georges Pompidou European Hospital, APHP, Paris Descartes University

Human papillomavirus (HPV) RNA chromogenic in situ hybridization is considered to be one of the gold standards for active human papillomavirus infection detection within tumors. It allows the visualization of HPV E6-E7 mRNA expression with localization and semiquantitative evaluation of its signal.