The Jackson Laboratory for Genomic Medicine

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Christopher Terranova¹, Ming Tang¹, Elias Orouji¹, Mayinuer Maitituoheti¹, Ayush Raman¹, Samirkumar Amin², Zhiyi Liu¹, Kunal Rai¹

¹Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, ²The Jackson Laboratory for Genomic Medicine

Here, we describe an optimized high-throughput ChIP-sequencing protocol and computational analyses pipeline for the determination of genome-wide chromatin state patterns from frozen tumor tissues and cell lines.