National Cancer Center
2 ARTICLES PUBLISHED IN JoVE
Cancer Research
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
Shiv Poojan 1, Han-Seong Kim 2, Ji-Woon Yoon 1, Hye Won Sim 1, Kyeong-Man Hong 1
1Omics Core Lab, Research Institute, National Cancer Center, 2Department of Pathology, Inje University Ilsan Paik Hospital
Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.
Cancer Research
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Ami Maruyama 1,2, Kenjiro Kami 3, Kazunori Sasaki 3, Hajime Sato 3, Yuzo Sato 1,2, Katsuya Tsuchihara 4, Hideki Makinoshima 1,2,4
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center
The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.
