National Cancer Center

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Shiv Poojan¹, Han-Seong Kim², Ji-Woon Yoon¹, Hye Won Sim¹, Kyeong-Man Hong¹

¹Omics Core Lab, Research Institute, National Cancer Center, ²Department of Pathology, Inje University Ilsan Paik Hospital

Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.

Cancer Research

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Ami Maruyama^1,2, Kenjiro Kami³, Kazunori Sasaki³, Hajime Sato³, Yuzo Sato^1,2, Katsuya Tsuchihara⁴, Hideki Makinoshima^1,2,4

¹Shonai Regional Industry Promotion Center, ²Tsuruoka Metabolomics Laboratory, National Cancer Center, ³Human Metabolome Technologies, Inc, ⁴Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center

The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.