Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Masaya Ikegawa^*1, Takashi Nirasawa^*2, Nobuto Kakuda¹, Tomohiro Miyasaka¹, Yuki Kuzuhara¹, Shigeo Murayama³, Yasuo Ihara⁴

¹Department of Life and Medical Systems, Doshisha University, ²Bruker Daltonics K.K., ³The Brain Bank for Aging Research, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, ⁴Graduate School of Brain Science, Doshisha University

Molecular imaging with matrix-assisted laser desorption/ionization-based imaging mass spectrometry (MALDI-IMS) allows the simultaneous mapping of multiple analytes in biological samples. Here, we present a protocol for the detection and visualization of amyloid β protein on brain tissues of Alzheimer's disease and cerebral amyloid angiopathy samples using MALDI-IMS.