Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass SpectrometryMasaya Ikegawa *1, Takashi Nirasawa *2, Nobuto Kakuda 1, Tomohiro Miyasaka 1, Yuki Kuzuhara 1, Shigeo Murayama 3, Yasuo Ihara 4
1Department of Life and Medical Systems, Doshisha University, 2Bruker Daltonics K.K., 3The Brain Bank for Aging Research, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 4Graduate School of Brain Science, Doshisha University
Molecular imaging with matrix-assisted laser desorption/ionization-based imaging mass spectrometry (MALDI-IMS) allows the simultaneous mapping of multiple analytes in biological samples. Here, we present a protocol for the detection and visualization of amyloid β protein on brain tissues of Alzheimer's disease and cerebral amyloid angiopathy samples using MALDI-IMS.
