Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

7 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Shouming Zhang^1,2,3, Xueping Gao^1,2,3, Genhui Xiao^1,2,3,4, Chengping Lu^1,2,3, Huochun Yao^1,2,3, Hongjie Fan^1,2,3,5, Zongfu Wu^1,2,3

¹College of Veterinary Medicine, Nanjing Agricultural University, ²Key Lab of Animal Bacteriology, Ministry of Agriculture, ³OIE Reference Lab for Swine Streptococcosis, ⁴School of Medicine, Tsinghua University, ⁵Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial meningitis.

Biochemistry

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Zhiping Zhang¹, Shuhang Fan¹, Zhiqiang Chen¹, Yanzhi He¹, Mengfei Huang¹, Li Ding¹, Yanyan Zhang⁵, Lin Chen¹, Xinyue Zhang^1,2,3,4

¹College of Bioscience and Biotechnology, Yangzhou University, ²Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, ³Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, ⁴Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ⁵The Testing Center, Yangzhou University

The derivation of a flavonol is crucial for its application in healthcare and the food industry. Here, we provide a detailed protocol for the biosynthesis of a flavonol from a flavanone and discuss the crucial steps and its advantages over other approaches.

Medicine

Osteoarthritis Pain Model Induced by Intra-Articular Injection of Mono-Iodoacetate in Rats

Jiaan Xu^1,2, Li Yan^1,2, Bo Yan^1,2, Li Zhou¹, Peijian Tong¹, Letian Shan¹

¹The First Affiliated Hospital, Zhejiang Chinese Medical University, ²College of Pharmaceutical Science, Zhejiang Chinese Medical University

This study describes the method of intra-articular injection of mono-iodoacetate in rats and discusses the resulting pain-related behaviors and histopathological changes, which provide references for future applications.

Medicine

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Chunlong Fu^*1,2, Yaqun Zhang^*1, Xin Wang¹, Shengcai Zhu¹, Huimin Hou¹, Shengjie Liu¹, Cheng Pang¹, Xinda Song¹, Jia Chen¹, Jianye Wang¹, Ming Liu^1,2

¹(Urology) Beijing Hospital, National Center of Gerontology, The Joint Unit of the Chinese Academy of Medical Sciences, ²Graduate School of Peking Union Medical College, Beijing Hospital

Here, we present a protocol to increase the surgical field of view and reduce the difficulty of total transperitoneal laparoscopic nephroureterectomy surgery by precutting the umbilical ligament before treating the terminal ureter.

Immunology and Infection

Use of Fimbrial Rod for F18ab Fimbriae⁺ STEC Colonization to Host Cells

Mingxu Zhou^*1,2, Qiangde Duan^*1,3, Yang Yang^1,3, Guoqiang Zhu^1,3

¹College of Veterinary Medicine, Yangzhou University, ²Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, ³Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Here we present a protocol to study the function of fimbriae in bacterial colonization.

Immunology and Infection

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Xia Meng^*1,2, Weiwei Cui^*1, Xianchen Meng¹, Jianye Wang^1,2, Jinqiu Wang³, Guoqiang Zhu^1,2

¹College of Veterinary Medicine, Yangzhou University, ²Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ³Department of Animal Husbandry and Veterinary Medicine, Beijing Agricultural Vocational College

An λ-Red-mediated recombination system was used to create a deletion mutant of a small non-coding RNA micC.

Immunology and Infection

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Pengpeng Xia^1,2,3, Siqi Lian^1,2,3, Yunping Wu^1,2,3, Li Yan^1,2,3

¹College of Veterinary Medicine (Institute of comparative medicine), Yangzhou University, ²Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ³Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University

The recombinant antibody protein expressed in pIRES2-ZSGreen1-rAbs-APN-CHO cells and monoclonal antibodies produced using traditional hybridoma technology can recognize and bind to the porcine aminopeptidase N (APN) protein.