Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular PericytesKarin Dreisig 1, Frank Wojciechowski Blixt 2, Karin Warfvinge 1,2
1Department of Clinical Experimental Research, Glostrup Research Institute, 2Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University
We demonstrate three different tissue preparation techniques for immunohistochemical visualization of rat retinal microvascular pericytes, i.e., cryo-sections, whole-mounts, and hypotonic isolation of the vascular network.
