S'identifier

Glostrup Research Institute

1 ARTICLES PUBLISHED IN JoVE

Biology

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Karin Dreisig¹, Frank Wojciechowski Blixt², Karin Warfvinge^1,2

¹Department of Clinical Experimental Research, Glostrup Research Institute, ²Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University

We demonstrate three different tissue preparation techniques for immunohistochemical visualization of rat retinal microvascular pericytes, i.e., cryo-sections, whole-mounts, and hypotonic isolation of the vascular network.