Brazilian Center for Research in energy and materials (CNPEM)
2 ARTICLES PUBLISHED IN JoVE
Environment
A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
Carolina C. Monte-Bello *1,2, Elias F. Araujo *1,3, Marina C.M. Martins 1, Valeria Mafra 4, Viviane C.H. da Silva 1,2, Viviane Celente 1, Camila Caldana 1,5
1Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in energy and materials (CNPEM), 2University of Campinas (UNICAMP), 3University of Viçosa (UFV), 4CTBE, CNPEM, 5Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Max Planck Partner Group
A simple, versatile, and low-cost in vitro hydroponic system was successfully optimized, enabling large-scale experiments under sterile conditions. This system facilitates the application of chemicals in a solution and their efficient absorption by roots for molecular, biochemical, and physiological studies.
JoVE Core
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Talita Miguel Marin 1, Nathalia de Carvalho Indolfo 4, Silvana Aparecida Rocco 1, Murilo de Carvalho 1,2, Marilia Meira Dias 1, Graziele Izalina Vasconcelos Bento 1, Leandro Oliveira Bortot 1, Desirée Cigaran Schuck 3, Márcio Lorencini 3, Eduardo Pagani 1
1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), 2Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 3Grupo Boticário, 4Institute of Biology, University of Campinas
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
